AffiAB® FbpB Antibody Pair (MAb Detection + MAb Capture)

Species Reactivity

Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)

Immunogen

Recombinant Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) FbpB protein

Immunogen Expression

E. coli

Immunogen Tag Info

N-terminal 6xHis-SUMO-tagged.

Deliverables

• 200 µg of immunogen (recombinant protein, purity > 85%);
• 25 µL of pre-immune mouse serum used in the generation of the detection antibody;
• 25 µL of pre-immune mouse serum used in the generation of the capture antibody;
• 1-2 mg of HRP or biotin-conjugated mouse monoclonal antibodies, purified by protein A/G (detection antibody);
• 5 mg of non-conjugated mouse monoclonal antibodies, purified by protein A/G (capture antibody).
  

Quality

• Antibody Purity > 95% (Tested by SDS-PAGE):
  The antibody has been purified to over 95% purity, as confirmed through SDS-PAGE analysis. This ensures minimal contamination and high specificity of the antibody for its intended target.
• Antibody Titer > 1:64,000 (Tested by Indirect ELISA):
  The antibody demonstrates a titer greater than 1:64,000, as determined by indirect ELISA. This indicates that the antibody is highly sensitive and capable of recognizing very low concentrations of the target antigen.
• Western Blot (WB) Positive with the Immunogen Protein:
  The antibody has been shown to bind specifically to the immunogen protein in Western blot analysis. This confirms that the antibody is specific to the immunogen and is capable of detecting the protein in complex mixtures.
• Sandwich ELISA Validation Data (Using Antigens as Standards):
  The antibody has been validated for use in sandwich ELISA assays, with antigens used as standards. This demonstrates the antibody's capability to capture and detect the target antigen in a robust and reliable manner, suitable for both qualitative and quantitative analysis.