AffiAB® FbpB Antibody Pair (PAb Detection + Mouse MAb Capture)

Species Reactivity

Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)

Immunogen

Recombinant Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) FbpB protein

Immunogen Expression

E. coli

Immunogen Tag Info

N-terminal 6xHis-SUMO-tagged.

Deliverables

• 200 µg of immunogen (recombinant protein, purity > 85%);
• 1 mL of pre-immune rabbit serum;
• 25 µL of pre-immune mouse serum;
• 1-2 mg of biotin or HRP-conjugated rabbit polyclonal antibodies, purified by antigen affinity (detection antibody);

• 5 mg of non-conjugated mouse monoclonal antibodies, purified by protein A/G (capture antibody).

Quality

• Antibody Purity > 95% (Tested by SDS-PAGE):
  The antibody has been purified to a high degree of purity, exceeding 95%. This was confirmed through SDS-PAGE analysis, a common method used to assess the purity of proteins and antibodies by separating them based on their molecular weight. The resulting gel analysis shows a single, distinct band corresponding to the target antibody, confirming minimal impurities.
• Antibody Titer > 1:64,000 (Tested by Indirect ELISA):
  The antibody's effectiveness was evaluated by its titer, which is greater than 1:64,000, as determined by an indirect enzyme-linked immunosorbent assay (ELISA). This high titer indicates that the antibody is present at a sufficiently high concentration to detect even trace amounts of the target antigen, ensuring sensitivity and specificity in assays.
• Western Blot (WB) Positive with the Immunogen Protein:
  The antibody shows a positive reaction in a Western blot analysis using the immunogen protein. This demonstrates that the antibody specifically binds to the intended target protein, confirming its specificity. The WB results provide evidence that the antibody can be used in applications requiring protein identification and validation.
• Sandwich ELISA Validation Data (Using Antigens as Standards):
  Sandwich ELISA validation tests were performed using specific antigens as standards. These assays confirmed the antibody’s capability to capture and detect the target antigen in a sandwich format. The validation ensures that the antibody performs well in both the capture and detection stages of an ELISA, making it suitable for quantitative and qualitative assays in various experimental conditions.